The objective of the experiment is to determine the specific varieties of pigments uncovered in a sugar beet leaf and also in a spinach sheet by using paper chromatography and two solvents: water soluble solvent and also lipid soluble solvent.Hypothesis
If a water dissolve solvent is present, then there will certainly be the motion of just the water dissolve pigments up the chromatography paper. This happens due to the fact that as the water travels up the paper, the water soluble pigments’ bonds come to be attracted to the water molecules because of dipole-dipole forces.
Through capillary action, this pigments will take trip up the paper until the bonds between the water and pigment end up being so weak that the pigment need to break the attraction and also leave itself imprinted at a details height increase the paper. ~ above the various other hand, Lipid dissolve pigments won’t move as result of the molecules’ lack of polarity. Therefore, lock will stay in the focused area uneven a lipid-soluble solvent is present.
If this lipid-soluble solvent is present, together opposed come the water-soluble solvent, then the lipid-soluble colours will relocate up the chromatography document instead of the water-soluble pigments. The exact same idea will happen. The lipid-soluble colors will travel up the record until their bonds between the water are so weak the they need to stop following the motion of the solvent, and also get inserted at a certain height over the original focused dot.
Spinach will have mainly chlorophyll A and also B since the leaf is completely green, contrasted to the beet leaf which is composed of both a red and green shade reflecting that various other pigments are present in this leaf.Calculations
|Carotene (yellow orange colour)|
|Rf = street pigment travelled = 9.0cm = 0.9375 distance solvent travelled 9.6cm|
|Xanthophylls (light yellow):|
|Rf = distance pigment travelled = 5.7cm = 0.59375 street solvent took trip 9.6cm|
|Chlorophyll A (blue green):|
|Rf = street pigment took trip = 3.7cm = 0.385416 distance solvent travelled 9.6cm|
|Chlorophyll B (yellow green):|
|Rf = distance pigment took trip = 2.5cm = 0.260416 distance solvent took trip 9.6cm|
|Rf = distance pigment took trip = 0.6cm = 0.0625 street solvent travelled 9.6cm|
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Together the solution travels up the paper, favor soluble pigments will certainly travel v the solvent until the bonds in between the solvent and pigment become so weak that it should break the attraction and also imprint chin a specific height increase the paper. Some determinants that affect document chromatography are: